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2025
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Determination of adenosine content by fully automated potentiometric titration
I. Introduction
Adenosine is an important intermediate in the synthesis of adenosine triphosphate (ATP), adenine, adenosine acid, and vidarabine, with the molecular formula C10H13N5O4.
It can directly enter the myocardium, undergo phosphorylation to generate adenosine acid, participate in myocardial energy metabolism, and also participate in the dilation of coronary arteries, increasing blood flow. It has physiological effects on the cardiovascular system and many other systems and tissues of the body. This experiment determined whether the adenosine content of a certain manufacturer's product met the standards.
The Shanghai Jiahang JH-T6 fully automatic potentiometric titrator was used to determine the endpoint by setting the potential jump point, and the content was measured.
II. Instruments and Reagents
2.1 Instruments
JH-T6 fully automatic potentiometric titrator, non-aqueous pH composite electrode, analytical balance, etc.
2.2 Reagents
Glacial acetic acid, 0.1 mol/L perchloric acid standard titrant
III. Experimental Methods
3.1 Experimental Procedure:
1) Determination of adenosine content: Accurately weigh 0.2 g of the sample (accurate to 0.0001 g) and place it in a dry titration cup. Add 40 mL of glacial acetic acid, heat gently to dissolve, place it on the potentiometric titration stage, turn on the stirrer to mix the sample completely, insert the composite electrode and titration head,
start the edited method, titrate with perchloric acid (0.100 mol/L) standard solution to the potential jump point, record the volume of standard titrant consumed, and perform a blank test simultaneously.
3.2 Instrument Parameters
The parameter settings for the JH-T6 fully automatic titrator are shown in Table 1:
表 1 滴定仪参数设置
IV. Results and Discussion
4.1 Experimental Results
The samples were tested, and the experimental results are shown in Table 2:
Table 2 Adenosine Content Test Results
4.2 Titration Spectrum
4.3 Conclusion
The adenosine content tested in this test using perchloric acid titration was 99.463%, which meets the production requirements. Furthermore, using instrumental judgment reduced human error and greatly improved the accuracy of the experiment. Potentiometric titration is a good choice for detecting this type of sample.
V. Precautions
1. The concentration of the perchloric acid titrant is greatly affected by the ambient temperature. It is recommended to standardize the concentration of the perchloric acid titrant before performing the titration experiment.
VI. References
[1] Chinese Pharmacopoeia 2020 Edition, Part II [M].
Keyword:
automated potentiometric titration
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