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2025

Determination of the content of asparttan by fully automatic potentiometric titration

1. Preface

Aspartan (C14H18N2O50), scientifically known as aspartan, is a non-carbohydrate artificial sweetener. When used as a sweetener, it does not cause a significant increase in blood sugar. Therefore, it is mainly used to make foods suitable for diabetic patients and can help lower blood sugar. However, studies have shown that Excessive intake may pose risks such as abnormal brain nerves and brain tumors. This plan utilizes the T960 fully automatic potentiometric titrator to determine the content of the active ingredient of the drug through non-aqueous titration with perchloric acid, to see if the content index meets the requirements for mass production. This scheme has a simple experimental process, takes less time, and avoids the subjective error caused by manual determination of the endpoint. It is a good choice for detecting the content of this type of drug.

2 Instruments and equipment

2.1 Instruments

JH-T7 fully automatic potentiometric titrator, composite PH electrode.

2.2 Reagents

Perchloric acid standard solution (0.1 mol/L), glacial acetic acid, anhydrous formic acid.

3 Experimental Methods

3.1 Experimental Procedures

Accurately weigh 0.15g(accurate to 0.0001g) of potassium hydrogen phthalate reference reagent, place it in a dry titration cup, add 50mL of glacial acetic acid solution, warm and stir until it is completely dissolved, place it on the stirring table, insert the electrode, titrate with perchloric acid titration solution until the potential suddenly crosses the endpoint, record the titration volume, and repeat three times. Meanwhile, conduct the blank titration experiment. Calculate the concentration of the titration solution.

(2) Accurately weigh 0.25g of the sample (accurate to 0.0001g) and place it in a dry titration cup. Add 3mL of formic acid and 50

mL glacial acetic acid, start stirring for 20 seconds to ensure complete dissolution of the sample, insert the electrode, start the prepared method, and titrate with the calibrated perchloric acid titration solution until the potential leap endpoint is reached. Record the titration volume, repeat this process three times, and simultaneously create a blank volume.

3.2 Parameter Settings

Titration mode: Dynamic titration		Stirring speed: 6
Electrode equilibrium time: 4 seconds		Pre-stirring time: 1 second
Electrode equilibrium potential: 1mv		liquid replenishment rate: 7
Minimum addition volume: 0.02mL.		Pre-titration addition volume: 0mL
Final volume: 10mL.		Stirring time after pre-titration: 1 second
Potential leap: 300		Pre-controlled mv value: None

4 Results and Discussion

4.1 Experimental Results

Sample name	sample number	titrant concentration (mol/L)	(g ) sampling quantity	titration volume V1 (mL)	blank volume where V0 (mL)	content (%)	average (%)	RSD(%)
Aspatan	1	0.1005	0.25245	8.224	0.06	95.650	95.592	0.114
	2		0.25295	8.224		95.461
	3		0.25485	8.303		95.666

Calculation formula:

In the formula:

m is the mass of the sample to be weighed;

V1 is the amount of titrant consumed;

V0 is the blank volume;

29.43 is the mass of C14H18N2O50 corresponding to 0.1 mol/L perchloric acid titrant, with the unit of mg

4.2 Atlas

4.3 Conclusion

The determination of the content of aspartan by the JH-T7 fully automatic potentiometric titrator has good repeatability. The determination results are all within its standard range, and the RSD is ≤0.5%. The T960 fully automatic potentiometric titrator fully meets the determination requirements of this sample.

Keyword:

Full Automatic Potentiometric Titrator

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